Generator

Part:BBa_K4242018:Design

Designed by: zhelin Jiang   Group: iGEM22_CUG-China   (2022-09-29)


RBS+FleQ+TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 886
    Illegal XhoI site found at 1339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 279
    Illegal NgoMIV site found at 1287
    Illegal NgoMIV site found at 1299
    Illegal NgoMIV site found at 1375
    Illegal NgoMIV site found at 1460
    Illegal AgeI site found at 688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 301
    Illegal SapI.rc site found at 1234
    Illegal SapI.rc site found at 1243


Design Notes

This is a composite part that uses FleQ and TT as an assembly we could use.


Source

Assemble RBS, FleQ coding region, and TT.

References